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rabbit anti trpm8 primary antibody  (Proteintech)


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    Proteintech rabbit anti trpm8 primary antibody
    Rabbit Anti Trpm8 Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+trpm8+primary+antibody/TRPM8+Antibody/pm39746519-95-48-52
    Average 94 stars, based on 10 article reviews
    rabbit anti trpm8 primary antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Optimization and Calibration of 384-well Kinetic Ca 2+ Mobilization Assays for the Human Transient Receptor Potential Cation Channels TRPM8, TRPV1, and TRPA1.
    Article Snippet: .. After 5 min at ambient temperature permeabilized cells were washed 1x with 50 μL of blocking buffer (0.1% Tween 20 in PBS) and then 50 μL of fresh blocking buffer was added to the wells and incubated at room temperature for 15 min. A 1:100 dilution (final) of rabbit anti-TRPM8 primary antibody (ProteinTech) was then added to wells using the Microflo bulk reagent dispenser and incubated for 2 h in the dark at room temperature. ..

    Incubation:

    Article Title: Optimization and Calibration of 384-well Kinetic Ca 2+ Mobilization Assays for the Human Transient Receptor Potential Cation Channels TRPM8, TRPV1, and TRPA1.
    Article Snippet: .. After 5 min at ambient temperature permeabilized cells were washed 1x with 50 μL of blocking buffer (0.1% Tween 20 in PBS) and then 50 μL of fresh blocking buffer was added to the wells and incubated at room temperature for 15 min. A 1:100 dilution (final) of rabbit anti-TRPM8 primary antibody (ProteinTech) was then added to wells using the Microflo bulk reagent dispenser and incubated for 2 h in the dark at room temperature. ..



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    (A) <t>TRPM8</t> mRNA expression by semi-quantitative PCR in superior mesenteric artery with endothelium removed (n = 3). C, control; P1, 1 μL prostate RT reaction; P2, 2 μL prostate RT reaction, Me, 2 μL mesenteric artery RT reaction. (B) Western blot analysis of TRPM8 protein expression in rat vascular tissue. Molecular weight of TRPM8 was 125 KDa. Bottom panel: β actin used to demonstrate protein loading (at 42kDa).
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    Image Search Results


    (A) TRPM8 mRNA expression by semi-quantitative PCR in superior mesenteric artery with endothelium removed (n = 3). C, control; P1, 1 μL prostate RT reaction; P2, 2 μL prostate RT reaction, Me, 2 μL mesenteric artery RT reaction. (B) Western blot analysis of TRPM8 protein expression in rat vascular tissue. Molecular weight of TRPM8 was 125 KDa. Bottom panel: β actin used to demonstrate protein loading (at 42kDa).

    Journal: PLoS ONE

    Article Title: TRPM8 Channel Activation Induced by Monoterpenoid Rotundifolone Underlies Mesenteric Artery Relaxation

    doi: 10.1371/journal.pone.0143171

    Figure Lengend Snippet: (A) TRPM8 mRNA expression by semi-quantitative PCR in superior mesenteric artery with endothelium removed (n = 3). C, control; P1, 1 μL prostate RT reaction; P2, 2 μL prostate RT reaction, Me, 2 μL mesenteric artery RT reaction. (B) Western blot analysis of TRPM8 protein expression in rat vascular tissue. Molecular weight of TRPM8 was 125 KDa. Bottom panel: β actin used to demonstrate protein loading (at 42kDa).

    Article Snippet: The membrane was blocked with 5% (wt/vol) milk in TBS containing 0.1% Tween 20 (TBST) for 1 h at room temperature, followed by overnight incubation at 4°C with the specific primary rabbit polyclonal antibody against TRPM8 (1:1000 dilution; Abcam, Cambridge, Massachusetts, United States).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Western Blot, Molecular Weight